Part:BBa_K5038024
PT7-LIRA_input05-T7TE
Usage and Biology
LIRA_input05 is the target to be detected by LIRA_H05, it will bind to the loop at the top of LIRA_H05, and then change its conformation so that the ribosome could bind to the RBS.
(Figure 1. LIRA_input05 binds to LIRA_H05)
This composite part is used to express input05 in E.coli to test the function of H05.
Characterization
T7 promoter could be induced by IPTG, so the intracellularly concentration of LIRA_input05 produces a large difference between the two conditions of IPTG presence/absence. This composite part is equipped by the following plasmids and co-transformed with the corresponding H05 plasmid. See BBa_K5038020 for details.
(Table 1. LIRA_input05 plasmids and the corresponding H05 plasmid)
No. | LIRA_input05 plasmids | corresponding H05 plasmid |
---|---|---|
1 | pCDFDuet-input05 | pET-15b-H05-linker-EGFP |
2 | pCOLADuet-input05 | pET-15b-H05-linker-EGFP |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |